. In addition to each of these written formats, you will need to submit data and any graphs before you begin your inquiry component. Due to the use of Hardy-Weinberg equation without the actual conditions, there are larger fluctuations in the allele frequencies. Remember that lab reports are individual assignments. Herron ©2002 Calculator Methods Methods below apply to all 6 cases in Table I below. Was your hypothesis correct, why or why not? Introduction Enzymes are proteins produced by living cells that act as catalysts, which affect the rate of a biochemical reaction. Once you have decided what might have caused error, you should explain how the error might have been avoided.
Salt concentration must be in an intermediate concentration. The purpose is also sometimes written as a separate section from the introduction. In Part 2, the materials used were 5 mL of catalase, a boiling water bath, 1 test tube, a test tube rack, 10 mL of 1. Since the heterozygous genotype was favored, the students noted it as having stabilizing selection. However, the pigments will move up the chromatography paper at different rates because they are not equally soluble to the solvent. Also briefly describe in your own words the important steps in the procedure, noting any changes that will be made to the procedure you referenced. A running Table of Contents is to be kept on the inside cover of the notebook.
They believed that all homozygous recessive genotypes would be lethal and that all homozygous dominant genotypes would have a 50% survival rate. Some of the factors that affect enzyme reactions are salt concentration, pH, temperature, substrate and product concentration, and activators and inhibitors. Record the number of adults of each genotype in your data table under the column Experimental. When the tip of the respirometer is submerged, no additional air will enter. For example, if the percentage of acetic acid in 10 samples of vinegar has to be calculated and then averaged, write out the calculation for only one of them, then mention that the calculation was repeated for 10 samples and give the average of all 10.
Information on each section is given below. We also marked where the solvent stopped, or the solvent front. For that reason, it is required that labs be read ahead of the first day of the week of class. Exercise 2D: An Enzyme-Catalyzed Rate of H2O2 Decomposition The baseline assay was reestablished following the directions of Exercise 2B. Please read over each of the following different. Materials and Equipment Provide a concise list of materials and equipment that are required to perform the experiment.
The same steps were repeated for the 30, 60, 120, 180, and 360 second cups, respectively. When members of a population migrate or move away from their habitat, various genes enter different communities and other populations. These pages will not be graded. Exercise 2B: The Baseline Assay This part of the lab required 10 mL of 1. Migration rates are originally 100% if there is no net movement. Organized the graphs or tables in the Appendix. Calculations: Calculate the results if appropriate.
In enzyme-catalyzed reactions, a substrate, the substance to be acted upon, binds to the active site on an enzyme to form the desired product. Since you may see this material again on an , it is best that you know it for yourself. In Case 1, the students hypothesized that only little or no fluctuations in the allele frequencies would result. Finally: Some pre and post lab questions are included with each lab. As O2 is used up, the pressure of gases inside the respirometer decreases.
What is the effect of the independent variable on the dependent variable? Respect for the autonomy of human beings is a. A diagram showing your particular set-up with labeled equipment may earn a few extra points! These guidelines concern autonomy, justice, beneficence, and nonmaleficence. It is set to something other than 0. Record the ending allele frequency in the data table. The homozygous recessive genotype is made lethal; therefore no adults are listed in the chart for this case. Lab Reports Lab reports will take one of three formats in this course depending on the type of lab and the form of inquiry follow-up that takes place.
This lab is helpful in conceptualizing the ideas, applications, and effects of osmosis and diffusion. Introduction: The purpose of this lab is to test the effect certain stimuli has on the behavior of a terrestrial isopod. Discussion and Conclusions Restate the purpose of the study, major findings, and support of the hypothesis by the data. Did you have a low percent yield or low percent error? In Case 3, with the addition of selection, the students hypothesized that there would be no 0 adults with genotype aa, and that therefore, the allele frequencies would have greater fluctuations. To narrow the possibilities, this specific trial will test whether potato bugs favor fresh water environments or salt water environments. Therefore, there was support that the Hardy-Weinberg Theory and the 5 cases for evolution were indeed accurate in their own particular cases 170 words.
As noticed in the Frequencies, there were only small fluctuations between the original 0. Then we used this data to find out which pigments were which color. Disposable laboratory notebook in the event of contamination or spills 3. Explain how the methods could be improved. Should you have been more careful with measurements? Each sample was assayed separately by placing each over a white sheet of paper. ¨ Procedure — this section should be brief. If any calculations were performed you should show them here.