Stop the watch and calculate the time from average of the tow capillary tubes. Purpuras can be due to 1. The tube gets easily filled by capillary action. After cleaning the forearm, make a venepuncture an draw 3 ml of blood in a silicon-sided glass or plast syringe. Patient discussion about clotting time Q. As soon as one can be tilted past the horizontal i. Cleanse the test area with the 70% alcohol and let it dry.
They are designated by Roman numerals, and an additional 'a' to indicate the activated state. Normal clotting time is 1-5 minutes. Provided are a blood sensor that is highly reliable and does not contaminate the interior of the main body of the device, a blood testing device and a blood analysis method. Procedures and Materials Used Procedure, Materials used and normal value D. Also, being at high altitudes may cause reticulocyte counts to rise, to help you adjust to the lower oxygen levels at high altitudes.
Touch the blood drop at the finger tip using one end of the capillary tube kept tilted downwards. Puncture to a depth of 3mm. Stop the time as soon as the bleeding stops. Select a site avoiding surface veins, bruises, and edematous areas. When the thread appears the stop watch is stoped.
Whole blood, when removed from the vascular system and exposed to a foreign surface, will form a solid clot. Bleeding time is prolonged in few disorders like: vascular lesions, platelet defect, severe liver disease, uremia and anti-coagulant drug administration. Blood is drawn into a test tube containing liquid sodium citrate, which acts as an anticoagulant by binding the calcium in a sample. Called the vascular phase, this is the first reaction of a blood vessel to damage. Tissue thromboplastin Plasma thromboplastin Needed in prothrombin conversion and plasma thromboplastin generation. Clotting time is defined as the time interval in between onset of bleeding and appearance of semisolid mass i. Disinfect the area of puncture with 70% alcohol.
Clotting time is also prolonged in conditions like vitamin K deficiency, liver diseases, disseminated intravascular coagulation, overdosage of anticoagulants etc. . Average value of the results in the 3 test tubes gives the clotting time. Whole blood clotting time Lee-white The whole blood clotting time is a rough measure of all intrinsic clotting factors in the absence of tissue factors. Techniques for more improved standardization of the test involve the introduction of glass beads and agents with similar surface characteristics to insure consistent maximal surface activation. Wipe off the first drop of blood 4. The clotting time is taken when the tubes can be title without spilling of their contents.
This can occur after a lot of bleeding, a move to a high altitude, or certain types of anemia. Normal value 1 - 7 minutes c. Make a sufficiently deep prick using a sterile lancet, so that blood comes out freely without squeezing. Hence, it is important to inform your healthcare provider of the complete list of medications including any herbal supplements you are currently taking. Less sensitive and now less often used than the activated time. Gently push the trigger, starting the stopwatch simultaneously. There are different methods of bleeding time: Duke's Method Ivy's Method Copley-Lalitch Immersion Test Aspirin Tolerance Test Quick's Test Bleeding Time Clotting Time Time required for a sample of blood to coagulate in vitro under standard conditions.
Materials 70% alcohol Sphygmomanometer Stopwatch Filter paper 2 - 3 sheets Sterile gauze Bleeding Time Procedures and Materials Used B. Normal Values Diseases related Bleeding Time Bleeding Time Procedures and Materials used. Clotting is one of the natural defense mechanisms of the body when injury occurs. Puncture to a depth of 3mm with a sterile disposable lancet 3. Plasma concentration is trace amount only. Ingestion of aspirin-containing compounds, anti inflammatory drugs, anticoagulants, and some antibiotics.
If bleeding time is more than 6 minutes, don't do the test anymore. Determine patient's first bleeding time using the Duke's method. Capillary Tube Method Dale and Laidlaw's method Sabraze's method a. This is repeated every 15 seconds, each time using a fresh portion of the filter paper, till bleeding stops. The time of no stain has come is noted properly; it is the bleeding time of the subject. Normal value is 3to 10 minutes.