By doing this test, Proteus vulgaris and were eliminated from the list. The plates showed two colonies with distinguishing colors; a white colored colony and an orange colored colony. After incubating for 48 hours, the agar plate turned yellow on one side and stayed red on the other. The reasons range from medical purposes, such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics to other purposes such as knowing the exact microorganism has to be used to make certain foods. Starch test was performed; it showed a clearing on the agar, indicating a positive result. Gram negative rods were observed. University of Oklahoma College of Medicine.
It looked over grown, so the same nutrient agar test was carried out several times. This type of colony are methyl red-negative lactose-fermenters. A Methyl Red test was performed and had negative results. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. In order for an individual to receive treatment for a certain bacterium, this bacterium must first be identified.
The Gram test concluded that the bacterium was a Gram negative rod. The… 3507 Words 15 Pages Kelsey Nolte Microbiology Otero Junior College Mr. This deduction was reached with several bits of evidence. No difficult problems occurred with the identification of B. A quadrant streak technique was used on a nutrient agar plate using the Alternative 4.
The other plate has gram + bacteriaThe gram-negative is pinkish red with a rod shaped , cocci. By comparative analysis of biochemical tests that were performed throughout the semester to find out its characteristics and determine the identification of bacterium out of four potential unknown was given. Scientist use Bacillus subtilis in order to promote genetic research and due to its highly genetic manipulability levels, it is easier to conduct testing on Swartzburg, 2009. Lastly, to determine which is a gram negative and gram positive, a gram stain test was used. The Methods and Results sections especially should be written in the past tense.
Then, it was concluded that only one bacterium was growing on the nutrient agar, so a gram stain was performed. One of the interesting features of Bacillus subtilis is that it is capable of forming endospores when its environment becomes hostile. The bacterium might not have mixed well with the Glycerol solution, creating an improper answer. The negative results explain that the bacterium did not have the enzyme urease, which breaks down urea. Wanting to be sure that Mannitol fermentation was not possible for this bacterium, a Mannitol tube was inoculated with the Gram positive bacteria and incubated. This activity includes the application of procedure , technique and skills related to what type of Agar to be use to grow culture, inoculating and sterilizing, reagents to apply, handling of samples away from contamination and the skills in gram staining , handling and use of microscope and the differentiate differential and selective test for gram-negative and gram-positive bacteria. Retrieved December 2, 2013 , , , ,.
While this does not initially seem logical, there are reasons behind it. After viewing it under a light microscope, pink rods were observed, confirming this. The purpose of this test is to determine whether or not a bacterium is able to utilize citrate as its sole carbon source McDonald et al. A gram stain was taken from a sample of the bacteria growing on the nutrient agar. Floyd, Jeanetta and Kennedy, Emily.
In fact, the strain 0157:H7, can be very threatening to humans, and in some cases can cause death. The other bacteria which was microscopically viewed as gram positive was missing in the incubator, another tube of sample was derived from the lab professor. Over a two week period, eight prepared types of test media were provided to identify the assigned unknown mixed cultures. This results in complete clearing of the blood around the colonies. The purpose of this test was to determine if the bacterium was able to ferment lactose and whether or not it produced a strong or weak acid, if at all McDonald et al.
Materials and… 896 Words 4 Pages Name: Danielle Title: Unknown Lab Report Introduction: There are many reasons for identifying an unknown bacterium. Like other bacteria in the Bacillaceae family, B. The Simmons Citrate showed the bacterium cannot solely use citrate as source of carbon. Gram + bacteria produce bubbles. Some of these organisms have distinct qualities that set them apart from one another, such as the exposure to certain environments. The starch test was used to identify which bacterium can produce the enzyme amylase. A negative casein test removed S.