If fresh stocks of dopamine were made and used soon it may not have been an issue. Ethanol is well known for its effects on both neurological and physiological processes in mammals. In my previous research, the effects of different water conditions i. After this, data collection is fairly quick- you could gather a reasonable amount of data in three 30-minute sessions or one 90-minute microscope marathon and be done. Also it is a type of water fleas, are used to study the effects of caffeine on heart rate. Also, the levels of nitrate remained steady at two of the three entry sites. In the experiment there will be several variables that I need to try and control, the temperature of the water is one of these because if the temperature is higher or lower on the day the results may end up being higher or lower, this would make my results inaccurate.
A German chemist, Friedrich Ferdinand Runge, discovered it in 1819. Therefore as the concentration of caffeine increases so will the heart rate of the Daphnia. To relate effects in Daphnia to effects in humans, it would be interesting to know how caffeine concentration in the water relates to caffeine concentration inside individual Daphnia? We used a microscope equipped with computer-aided real-time imaging capability Magnus Live usb camera viewer, version 2. The threat is in the risk of losing these irreplaceable structures forever. What is the reason of this sudden switch over? We have shown that much lower concentrations of ethanol kill Daphnia in a rather short period of time.
Despite this, aspirin has beneficial effects in the heart. It is considered ethical to use invertebrates such as daphnia rather than large organisms e. Music has an arousal effect which is related to its frequency and tempo. We wanted to see if caffeine affected the daphnia's heart rate in the same way. If not, it might be wise to use diagrams found on line to help your audience visualize Daphnia anatomy. Every day I counted the populations and checked the pH and temperature.
Safety: Handle cavity slide carefully to prevent breakage. Hypothesis: Daphnia subjected to a caffeine solution will show a rise in heart rate as caffeine is a stimulant that speeds up the body affecting the nervous system and rate in which the nerve pulses are sent and received in humans. Also, it would be more convincing with the following: 1 report the total number of animals studied in each condition. Our environment is affected by many pollutants on a daily bases forming a threat to natural stability. It is a drug that acts as a stimulant in humans and causes a raised heart rate, and is used to give more energy in drinks such as tea and coffee, add flavour to drinks such as coke, and in weight-loss foods. You can change the regional settings on your computer so that the spreadsheet can be interpreted correctly. This suggests that the culture of Daphnia used in the experiment could have contained shrimp that are different age and size.
Repeat all the above steps for the control pond water. During both of my experiments, my raw data was documented in a notebook and then transferred to a computer for analysis at the end of the experiments. Caffeine can be consumed from tea leaves, and coffee. I think they should clarify the followings points: 1. It is transparent and its heart is visible without the need for dissection. The outer carapace of the individuals is transparent, so you can see through to the internal organs see Figure 1, below.
I collected the water at both the entry and exit points of the creeks. I examined standard measurements of heart rate and birthrate and documented this data. How were drug solutions prepared? Keep in mind that is entirely possible that your curve will look different from all three of the curves in Figure 2. The divorce rate is increasing rapidly. The first container was our control with no caffeine, the second was filled with a.
Thanks to the British Pharmacological Society for providing the teaching notes on this practical. Evaluation Reliability: My results appear fairly reliable because there is an obvious pattern occurs and it supports my prediction. Caffeine, which is a stimulant drug rttused in coffee, tea, Red Bull and Pro-plus, causes increased amounts of stimulatory neurotransmitters to be released. Although I tested each concentration 10 times, there is still a lot of variability of the results. Referee Expertise: Cardiovascular physiology, neurophysiology, animal models Many thanks for your valuable suggestions. Dopamine synergizes with caffeine to increase the heart rate of Daphnia. The two highest concentrations from the pilot experiment can be excluded from the full experiment.
I will use the same type of caffeine and record the amount of time the daphnia spend in the caffeine before the counting begins, and try to keep this to a similar range. Many tissues from multicellular invertebrates do not require supplemental oxygen or complex media, unlike vertebrate tissues. The variability in the other contents are at 168 caffeinated coffee and 108 decaffeinated coffee This could perhaps be the effect of other chemicals in caffeine which Daphnia are responsive to. Clear scum from the surface of the water; but leave debris that sinks to the bottom — it may contain Daphnia eggs. The control for this experiment is a solution with no caffeine which allows us to see if the change in heart rate is due to the caffeine or other factors. Brush Creek's levels of nitrate, chlorine and phosphate were comparable to the levels in the other creeks, but the concentration of nitrate and the pH level at Brush Creek were the highest of the sites tested. The heart beat of Daphnia is very rapid, so count the beats by making dots on a piece of paper in the shape of a letter S.